CovaChem's LCMS Heptafluorbutyric acid is well suited as a chromatography additive for use in HPLC and LCMS applications. Heptafluorobutyric acid (HFBA) is typically beneficial as a separations enhancer, and is a moderately strong ion pairing agent, which can be a very effective way of gaining good analyte separation and sharp peaks in HPLC applications. This moderately strong ion pairing effect can unfortunately reduce ionization efficiencies (compared to other acid additives, such as Formic acid (11202) and Acetic acid (11201). This reduction of ionization efficiency can often be overcome by the incorporation of the post-column addition a “TFA fix” reagent, such as a highly refined (2-(2-methoxyethoxy) ethanol (CovaChem 11207) or LCMS Grade Propionic acid (CovaChem 11207). Please feel free to contact our technical support for more information on this subject.
CovaChem's LCMS Grade HFBA comes in two convenient package sizes (10 x 1 mL Ampules and 25 mL bottles). The 1 mL Ampules of LCMS Grade HFBA are useful for quickly preparing 1 Liter bottles of 0.1% HFBA solutions in either water or other organic solvents.
Package Sizes: 10 x 1 mL and 25 mL
Product Numbers: 11203-10x1 or 11203-25
2-Iminothiolane hydrochloride (Traut's Reagent) is used to convert primary amine (-NH2) groups to sulfhydryl groups (-SH) at pH 7 to 10. Traut's reagent (2-IT) is a water soluble molecule that is easy to use and creates a wide range of flexibility to the researcher.
3-Maleimidopropionic acid N-hydroxysuccinimide ester (BMPS) is a heterobifunctional crosslinker with a spacer length of 6.9 Å. BMPS contains an amine reactive, NHS ester, and a maleimide group which is reactive towards sulfhydryl functional groups.
Citraconic Anhydride (Methylmaleic anhydride) is used to block primary amine (-NH2) groups at pH 7 to 9. Citraconic anhydride reacts with primary amines to create a reversible amide linkage, which can be reversed under acidic conditions or in the presence of hydroxylamine.