CovaChem's LCMS Heptafluorbutyric acid is well suited as a chromatography additive for use in HPLC and LCMS applications. Heptafluorobutyric acid (HFBA) is typically beneficial as a separations enhancer, and is a moderately strong ion pairing agent, which can be a very effective way of gaining good analyte separation and sharp peaks in HPLC applications. This moderately strong ion pairing effect can unfortunately reduce ionization efficiencies (compared to other acid additives, such as Formic acid (11202) and Acetic acid (11201). This reduction of ionization efficiency can often be overcome by the incorporation of the post-column addition a “TFA fix” reagent, such as a highly refined (2-(2-methoxyethoxy) ethanol (CovaChem 11207) or LCMS Grade Propionic acid (CovaChem 11207). Please feel free to contact our technical support for more information on this subject.
CovaChem's LCMS Grade HFBA comes in two convenient package sizes (10 x 1 mL Ampules and 25 mL bottles). The 1 mL Ampules of LCMS Grade HFBA are useful for quickly preparing 1 Liter bottles of 0.1% HFBA solutions in either water or other organic solvents.
Package Sizes: 10 x 1 mL and 25 mL
Product Numbers: 11203-10x1 or 11203-25
Biotin is a compound used to biotinylate proteins with the addition of a zero length cross linking reagent. D-Biotin has several biology research applications, where the avidin biotin interaction is utilized.
Hydroxylamine Hydrochloride (Hydroxylamine HCl) is a widely used reducing agent used in biomolecular applications. Hydroxylamine HCl is used for reducing EGS crosslinkers and deprotecting SATA protected proteins.
Sulfo-SMCC is a water soluble heterobifunctional crosslinker used in antibody conjugation reactions. One end of Sulfo-SMCC contains a sulfo-NHS moiety that is reactive towards primary amines (-NH2) groups. The other end of Sulfo-SMCC is reactive towards sulfhydryl groups. Sulfo-SMCC is often used to crosslink antibodies to other peptides or proteins, or forming maleimide activated antibodies.