CovaChem's LCMS Heptafluorbutyric acid is well suited as a chromatography additive for use in HPLC and LCMS applications. Heptafluorobutyric acid (HFBA) is typically beneficial as a separations enhancer, and is a moderately strong ion pairing agent, which can be a very effective way of gaining good analyte separation and sharp peaks in HPLC applications. This moderately strong ion pairing effect can unfortunately reduce ionization efficiencies (compared to other acid additives, such as Formic acid (11202) and Acetic acid (11201). This reduction of ionization efficiency can often be overcome by the incorporation of the post-column addition a “TFA fix” reagent, such as a highly refined (2-(2-methoxyethoxy) ethanol (CovaChem 11207) or LCMS Grade Propionic acid (CovaChem 11207). Please feel free to contact our technical support for more information on this subject.
CovaChem's LCMS Grade HFBA comes in two convenient package sizes (10 x 1 mL Ampules and 25 mL bottles). The 1 mL Ampules of LCMS Grade HFBA are useful for quickly preparing 1 Liter bottles of 0.1% HFBA solutions in either water or other organic solvents.
Package Sizes: 10 x 1 mL and 25 mL
Product Numbers: 11203-10x1 or 11203-25
Ethylene glycol bis(succininic acid) N-hydroxysuccinimide ester) is a cleavable, homobifuntional crosslinker that contains two NHS functional groups. The NHS functional groups of EGS are selectively reactive towards primary amines at pH 7 to 9. EGS is cleavable with hydroxylamine at pH 8.5. EGS is often used as a cleavable option when disulfide containing crosslinkers, such as DSP (CovaChem 13304) and DTSSP (CovaChem 13303) are not an option due to the desire to retain disulfide networks of a protein intact.
Hexafluoroisopropanol (HFIP) is also called 1,1,1,3,3,3-Hexafluoro-2-propanol or HFIP. HFIP is an acylation reagent that is used to introduce fluorine atoms into a compound and improve volatility. This is useful for Gas Chromatography (GC) applications and GCMS applications.
Succinimidyl 6-[3-(2-pyridyldithio)propionamido] hexanoate (LC-SPDP) is a heterobifunctional crosslinker that is amino reactive on one end and sulfhydryl reactive on the other end. LC-SPDP contains a long spacer arm (15.7 Å)for increased molecular flexibility and a reducible disulfide, which is allows for cleavage of the crosslinker after crosslink formation.