Trifluoroacetic Acid (LCMS Grade TFA)

Product Name: LCMS Grade TFA (Trifluoroacetic acid)

Alternative Names: LCMS TFA; LCMS TFA Amps; TFA Ampules; Trifluoroacetic acid; TFA Acid; CAS# 76-05-1; Perfluoric acid; 2,2,2-Trifluoroacetic acid; HPLC TFA

CovaChem's LCMS Grade Trifluoroacetic acid (TFA) is well suited as a chromatography additive for difficult to separate analytes in HPLC and LCMS applications. CovaChem's LCMS TFA is an ideal additive for separations of peptides in peptide synthesis applications. Trifluoroacetic acid is widely used to separate both protected amino acid chains and deprotected peptides. Due to the amino and carboxylate groups having vastly different pka values, synthetic peptides can be particularly challenging to separate, The HPLC separation of peptides is much more easily achieved with 0.1% Trifluoroacetic acid being present in the mobile phase.

Trifluoroacetic acid (TFA) is typically beneficial as a separations enhancer, and is a moderately strong ion pairing agent, which can be a very effective way of gaining good analyte separation and obtaining sharp peaks in HPLC applications. Although TFA is and excellent low pH buffer for HPLC applications, this ion pairing effect has some negative impact on ionization efficiencies (compared to other acid additives, such as Formic acid (CovaChem 11202) and Acetic acid (CovaChem 11201. CovaChem offers several remedies for the impact of 0.1% TFA on ionization, in serving highly refined products that reverse this effect. This reduction of ionization efficiency can be overcome by incorporating a post-column addition of a “TFA fix” reagent, such as CovaChem's UltraPure (2-(2-methoxyethoxy) ethanol (CovaChem 11207) or LCMS Grade Propionic acid (CovaChem 11206). These products are easy to employ, and allow LCMS to be performed on peptides and proteins without altering HPLC methods that employ 0.1% TFA. These tfa fix reagents have saved countless hours of hplc method development and validation hours. Please feel free to contact our technical support for more information on this subject.

CovaChem's LCMS Grade TFA comes in two convenient package sizes (10 x 1 mL Ampules and 25 mL bottles). The 1 mL Ampules of LCMS Grade TFA are useful for quickly preparing 1 Liter bottles of 0.1% TFA solutions in either water or other organic solvents.

Package Sizes: 10 x 1 mL and 25 mL

Product Numbers: 11204-10x1 or 11204-25

Specifications

Appearance: Clear liquid

Purity: is ≥ 99.5%

Storage: Room Temperature

Properties

Physical State: Liquid

CAS #: 76-05-1

Chemical Formula: C2HF2O2

Molecular Weight: 114.02 amu

pKa: 0.23

Boiling Point: 72.4 °C (Lit)

Density: 1.489 g/mL

Tariff Code: 2915.90.5000

Important Notes:

(1) A minimum of two (2) packages is required if ordering 11204-10x1.

(2) Hazmat charge INCLUDED in the shipping price at checkout.

(3) Due to the weight and hazards associated with this product, not all shipping options are available for this product. After receipt of order, a customer service representative may contact you to modify shipping options and pricing.