NHS-SS-Biotin Product Description
NHS-SS-Biotin is a cleavable, long chain amine reactive biotinylation reagent. NHS-SS-Biotin reacts efficiently with compounds containing primary amine (-NH2) residues, including small molecules, proteins, antibodies and other biomolecules.
NHS-SS-Biotin contains a biotin tag, an elongated spacer arm with a reducible disulfide (S-S) and an active NHS ester. This biotinylation compound should be dissolved in a high quality, molecular biology grade, anhydrous solvent, such as DMSO (CovaChem 18252) or DMF (CovaChem 18251) prior to adding to final reaction buffer.
NHS-SS-Biotin labeled proteins can be easily cleaved by exposure to a solution containing TCEP (CovaChem 11303) or DTT (CovaChem 11302).
Package Sizes: 100 mg and 1 gram
Product Numbers: 14204-100; 14204-1
Product Name: NHS-SS-Biotin
Alternative Names: Succinimidyl 2-(biotinamido)-ethyl-1-3’-dithiopropionate; NHS-SS-(+)-Biotin; CAS# 142439-92-7; C19H28N4O6 S3
Appearance: White to Off-White Solid
Purity: is ≥ 90% (Quantitative NMR)
Storage: 4 °C, protected from moisture
PropertiesPhysical State: Solid
Chemical Formula: C19H28N4O6 S3
Molecular Weight: 504.65
Spacer length: 24.3 Å