Biotinylation Compounds

  • Biotin-Hydrazide

    Biotin Hydrazide Product Description

    Biotin-Hydrazide is a protein modification reagent commonly used to target glycans and glycoproteins. Biotin-Hydrazide efficiently reacts with sodium periodate (CovaChem 11310-25) oxidized sugar residues or aldehydes by forming stable hydrazone linkages under mild reaction conditions at pH values of 5 to 7.

    CDI Reaction Mechanism

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 14201-100; 14201-1

    Product Name: Biotin Hydrazide

    Alternative Names: Hydrazide-Biotin; Biotin-Hz; C10H18N4O2S; 666640-86-6


    Specifications

    Appearance: White Solid

    Purity: is ≥ 92%

    Storage: 4 °C, protected from moisture

     

    Properties

    Physical State: Solid

    CAS#: 666640-86-6

    Chemical Formula: C10H18N4O2S

    Molecular Weight: 258.34

    Spacer length: 15.7 Å

    Melting Point: 242 - 247 °C (Lit)

    Tariff Code: 2930.90.9190


    Additional Product Information:
    Biotin Hydrazide Product Information Bulletin

    $38.00
  • Biotin-LC Hydrazide

    Hydrazide-LC-Biotin Product Description

    Hydrazide-LC-Biotin (Biotin-X-Hydrazide) is a long chain protein modification reagent commonly used to target glycoproteins and glycans. Biotin-LC Hydrazide efficiently reacts with sodium periodate (CovaChem 11310-25) oxidized sugar residues or aldehydes, forming stable hydrazone linkages under mild reaction conditions at pH values of 5 to 7.

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 14209-100; 14209-1

    Product Name: Biotin-LC Hydrazide

    Alternative Names: Hydrazide-LC-Biotin; Biotin-LC-Hz; Biotin Long Chain Hydrazide; Biotin-X-Hydrazide; C16H29N5O3S; 109276-34-8


    Specifications

    Appearance: White Solid

    Purity: is ≥ 92%

    Storage: 4 °C, protected from moisture

     

    Properties

    Physical State: Solid

    CAS#: 109276-34-8

    Chemical Formula: C16H29N5O3S

    Molecular Weight: 371.50

    Spacer length: 24.7 Å

    Tariff Code: 2930.90.9190


    $118.00
  • Biotin-NHS (CAS 35013-72-0)

    Biotin NHS Product Description

    Biotin-NHS (N-Hydroxysuccinimidobiotin) is a widely utilized amine reactive biotinylation reagent. NHS-biotin reacts efficiently and irreversibly with a wide range of compounds containing a primary amine (-NH2) residue, including proteins, antibodies and other biomolecules. This simple biotinylation compound is membrane permeable, making it well suited for intracellular protein labeling.

    NHS-Biotin is uncharged and contains a short alkyl spacer arm, giving it limited water solubility. This compound should be dissolved in a molecular biology grade, anhydrous solvent, such as DMSO (CovaChem 18252) or DMF (CovaChem 18251), prior to addition to aqueous protein containing solutions.

    Biotin NHS Reaction Mechanism

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 14202-100; 14202-1

    Product Name: Biotin NHS

    Alternative Names: d-Biotin NHS; BNHS; Biotinyl-N-hydroxysuccinimde; N-Hydroxysuccinimidobiotin; NHS-Biotin; N-Succinimidyl D-biotinate; C14H15N3O5S; 35013-72-0


    Specifications

    Appearance: White Solid

    Purity: is ≥ 95%

    Storage: 4 °C, protected from moisture

     

    Properties

    Physical State: Solid

    CAS#: 35013-72-0

    Chemical Formula: C14H15N3O5S

    Molecular Weight: 341.38

    Spacer length: 13.5 Å

    Melting Point: 210 - 218 °C (Lit)

    Tariff Code: 2934.99.9000



    Additional Product Information:
    Biotin-NHS Product Information Bulletin

    $35.00
  • D-Biotin

    D-Biotin Product Description

    D-Biotin, also known as Vitamin H, is a compound found inside cells. Biotin is critical to several biological pathways in the body. This compound has found great utility in life science research due to its strong interaction with avidin and streptavidin. This interaction allows for biotinylated compounds, such as biotinylated enzymes or biotin labeled antibodies, to be easily pulled out of a mixture for isolation or further purification.

    The carboxylic acid end of D-Biotin can be used to react with directly with amine containing proteins through the use of EDC, CDI or other zero length coupling agent. Alternatively, CovaChem also carries a full line of reagents specifically designed to biotinylate antibodies or other proteins.

    CovaChem's Biotin (CAS# 58-85-5) is among the highest purity in the industry. This product is molecular biology grade, and is intended for sensitive biological applications and for high purity synthesis of structurally similar compounds.

    Package Sizes: 100 mg, 1 gram and 5 grams

    Product Numbers: 14208-100, 14208-1 and 14208-5 (Guaranteed In Stock!)

    Product Name: D-Biotin

    Alternative Names: D-Biotin; (+)-Biotin; Vitamin H; Bios II; Coenzyme R; Vitamin B7; Hexhydro-2-oxo-1H-thieno (3,4-d) imidazole-4-pentanoic acid; C10H16N2 O3S

    Specifications

    Appearance: White Solid

    Purity: is ≥ 99%

    Storage: 4 °C, protected from moisture

    Properties

    Properties Physical State: Solid

    CAS#: 58-85-5

    Chemical Formula: C10H16N2 O3S

    Molecular Weight: 244.31

    Spacer length: 13.5 Å

    Melting Point: 231-233 °C (Lit)

    Tariff Code: 2936.29.5030

    $27.00
  • NHS-LC-Biotin (NHS-X-Biotin)

    NHS-LC-Biotin Product Description

    NHS-LC-Biotin (NHS-X-Biotin) is a long chain amine reactive biotinylation reagent. NHS-LC-Biotin reacts efficiently and irreversibly with a wide range of compounds containing primary amine (-NH2) residues, including proteins, antibodies and other biomolecules.

    NHS-LC-Biotin contains a biotin moeity, an elongated alkyl chain spacer arm and an active NHS ester. This biotinylation compound is membrane permeable, making it well suited for intracellular protein labeling. This compound should initially dissolved in a high quality, molecular biology grade, anhydrous solvent, such as DMSO (CovaChem 18252) or DMF (CovaChem 18251). Once reconstituted, this compound can be used in organic solvent, or amine free, near neutral buffer, such as PBS, pH 7.2 (CovaChem 19213).

    NHS-LC-Biotin Reaction Mechanism

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 14203-100; 14203-1

    Product Name: NHS-LC-Biotin

    Alternative Names: Succinimidyl-6-(biotinamido)hexanoate; NHS-LC-Biotin; NHS-X-Biotin; Biotin-LC-NHS; LC NHS-(+)-Biotin; C20H29N4O5S; 72040-63-2


    Specifications

    Appearance: White Solid

    Purity: is ≥ 90% (Quantitative NMR)

    Storage: 4 °C, protected from moisture

     

    Properties

    Physical State: Solid

    CAS#: 72040-63-2

    Chemical Formula: C20H29N4O5S

    Molecular Weight: 454.54

    Spacer length: 22.4 Å

    Tariff Code: 2934.99.9000


    $109.00
  • NHS-SS-Biotin

    NHS-SS-Biotin Product Description

    NHS-SS-Biotin is a cleavable, long chain amine reactive biotinylation reagent. NHS-SS-Biotin reacts efficiently with compounds containing primary amine (-NH2) residues, including small molecules, proteins, antibodies and other biomolecules.

    NHS-SS-Biotin contains a biotin tag, an elongated spacer arm with a reducible disulfide (S-S) and an active NHS ester. This biotinylation compound should be dissolved in a high quality, molecular biology grade, anhydrous solvent, such as DMSO (CovaChem 18252) or DMF (CovaChem 18251) prior to adding to final reaction buffer.

    NHS-SS-Biotin labeled proteins can be easily cleaved by exposure to a solution containing TCEP (CovaChem 11303) or DTT (CovaChem 11302).

    NHS-SS-Biotin Reaction Mechanism

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 14204-100; 14204-1

    Product Name: NHS-SS-Biotin

    Alternative Names: Succinimidyl 2-(biotinamido)-ethyl-1-3’-dithiopropionate; NHS-SS-(+)-Biotin; CAS# 142439-92-7; C19H28N4O6 S3


    Specifications

    Appearance: White to Off-White Solid

    Purity: is ≥ 90% (Quantitative NMR)

    Storage: 4 °C, protected from moisture

     

    Properties

    Physical State: Solid

    CAS#: 142439-92-7

    Chemical Formula: C19H28N4O6 S3

    Molecular Weight: 504.65

    Spacer length: 24.3 Å


    Additional Product Information:
    NHS SS Biotin Product Information Bulletin
    NHS SS Biotin MSDS

    $128.00
  • Sulfo-NHS Biotin

    Sulfo-NHS Biotin Product Description

    Sulfo NHS Biotin (N-Hydroxysulfosuccinimidobiotin) is a water soluble, amine reactive biotinylation reagent. Sulfo NHS-biotin reacts efficiently and irreversibly with a wide range of compounds containing a primary amine (-NH2) residue, including proteins, antibodies and other biomolecules.

    Sulfo NHS-Biotin contains a short alkyl chain spacer arm and an active Sulfo NHS ester. This biotinylation compound is membrane impermeable, making it ideally suited for cell membrane protein labeling. This compound can be dissolved directly in aqueous buffer, such as PBS (CovaChem 19213) or reconstituted in higher concentrations in a high quality, molecular biology grade, anhydrous solvent, such as DMSO (CovaChem 18252) or DMF (CovaChem 18251).

    Sulfo NHS Biotin Reaction Mechanism

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 14205-100; 14205-1

    Product Name: Sulfo NHS Biotin

    Alternative Names: d-Biotin Sulfo NHS; SNHS Biotin; Biotinyl-N-hydroxysulfosuccinimde; N-Hydroxysulfosuccinimidobiotin; Sulfo NHS-Biotin; N-Sulfosuccinimidyl D-biotinate; C14H18N3O8S2Na; 119616-38-5


    Specifications

    Appearance: White Solid

    Purity: is ≥ 90%

    Storage: 4 °C, protected from moisture

     

    Properties

    Physical State: Solid

    CAS#: 119616-38-5

    Chemical Formula: C14H18N3O8S2Na

    Molecular Weight: 443.43

    Spacer length: 13.5 Å

    Tariff Code: 2934.99.9000


    $128.00
  • Sulfo-NHS LC-Biotin (Sulfo NHS-X-Biotin)

    Sulfo NHS-LC-Biotin Product Description

    Sulfo NHS-LC-Biotin (Sulfo NHS-X-Biotin) is a water soluble, long chain amine reactive biotinylation reagent. Sulfo NHS-LC-Biotin reacts efficiently and irreversibly with a compounds containing primary amine (-NH2) residues, including small molecules, proteins, antibodies and other biomolecules.

    Sulfo NHS-LC-Biotin contains a biotin group, an elongated alkyl spacer arm and an active Sulfo NHS ester. This biotinylation compound is membrane impermeable, making it well suited for cell surface protein biotinylation. This compound can be added directly to aqueous buffered reactions or can be dissolved in a high quality, molecular biology grade, anhydrous solvent, such as DMSO (CovaChem 18252) or DMF (CovaChem 18251) prior to adding to final reaction buffer.

    Sulfo NHS-LC-Biotin Reaction Mechanism

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 14206-100; 14206-1

    Product Name: Sulfo NHS-LC-Biotin

    Alternative Names: Sulfosuccinimidyl-6-(biotinamido)hexanoate; Sulfo-NHS-LC-Biotin; Sulfo-NHS-X-Biotin; Biotin-LC-Sulfo NHS; Sulfo NHS-LC-(+)-Biotin; CAS# 127062-22-0; C20H29N4NaO9 S2


    Specifications

    Appearance: White Solid

    Purity: is ≥ 90% (Quantitative NMR)

    Storage: 4 °C, protected from moisture

     

    Properties

    Physical State: Solid

    CAS#: 127062-22-0

    Chemical Formula: C20H29N4NaO9 S2

    Molecular Weight: 556.59

    Spacer length: 22.4 Å

    Tariff Code: 2934.90.9190


    $138.00
  • Sulfo-NHS-SS-Biotin

    Sulfo NHS-SS-Biotin Product Description

    Sulfo NHS-SS-Biotin is a reducible, long-chain amino reactive biotinylation agent. Sulfo NHS-SS-Biotin reacts with molecules containing primary amine (-NH2) residues, including small molecules, proteins, antibodies and other biomolecular compounds.

    Sulfo NHS-SS-Biotin contains a biotin moeity, an elongated spacer arm with a reducible disulfide (S-S) and an active, amine reactive NHS ester. This biotinylation reagent can be added directly to aqueous reactions, or can be dissolved in a high quality, molecular biology grade, anhydrous solvent, such as DMSO (CovaChem 18252) or DMF (CovaChem 18251) prior to adding to final aqueous reaction buffer.

    Sulfo NHS-SS-Biotin labeled proteins can be cleaved through the fascile reaction with TCEP (CovaChem 11303) or DTT (CovaChem 11302), allowing for quantitative release of biotin tag.

    Sulfo NHS-SS-Biotin Reaction Mechanism

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 14207-100; 14207-1

    Product Name: Sulfo NHS-SS-Biotin

    Alternative Names: Sulfosuccinimidyl 2-(biotinamido)-ethyl-1-3’-dithiopropionate; Sulfo NHS-SS-(+)-Biotin; C19H27N4O9 S4Na


    Specifications

    Appearance: White to Off-White Solid

    Purity: is ≥ 90% (Quantitative NMR)

    Storage: 4 °C, protected from moisture

     

    Properties

    Physical State: Solid

    Chemical Formula: C19H27N4O9 S4Na

    Molecular Weight: 606.69

    Spacer length: 24.3 Å


    $148.00