LCMS and HPLC Additives


LCMS Mobile Phase Chromatography additives can make a drastic improvement in both separations and ionization. The level of product refinement is critical to the successfully implementing mobile phase additives into your chromatagraphy and spectrometry applications. CovaChem's LCMS and HPLC additives are some of the most highly refined chemicals in the industry, making them ideally suited for sensitive HPLC and LCMS applications.

One of the pre-requisites for LCMS buffers is that the mobile phases prepared, and all mobile phase additives, should be volatile. This is one of the reasons why buffers such as Sodium Phosphate are not commonly relied upon for LCMS applications. Although many inorganic buffers lend themselves to excellent separations and effectively mask charged groups which cause poor peak shape (ie. tailing and fronting), they are typically unsuitable for LCMS due to their lack of volatility.

CovaChem offers a variety of UltraPure LCMS Grade volatile buffer additives that are compatible with LCMS, HPLC, and proteomics applications, including: Trifluoracetic acid (TFA), Formic acid plus TFA, Acetic acid, Formic acid, Heptafluorobutyric acid (HFBA), Triethylamine (TEA), Propionic acid

In both LCMS and HPLC applications, it is critical that mobile phases consistent of very highly refined components that are free of interfering contaminants, metal contaminants, and other non-volatiles. Consistent composition of mobile phases is also important, as small variations in concentration and pH can be the difference between adequate separation or not. Not only does CovaChem carry the highest quality buffers for LCMS and HPLC applications, but it is conveniently packaged in multiple packaging sizes, including 1 mL ampules, for easily and accurately preparing one liter of 0.1 % buffers in either water or organic phases. CovaChem also offers custom ampule packaging, for your own mixture and volumes. Call today +1-815-315-1271 for more information.
  • LCMS Grade Acetic Acid (UltraPure)

    LC/MS Grade Acetic Acid Product Description

    CovaChem's LCMS Grade Acetic Acid is ideally suited as a chromatography additive for use in both HPLC and LCMS applications. Acetic acid is typically beneficial as a separations enhancer, and has a weak ion pairing effect in the mass spectrometer, making dissociation of from the analytes relatively easy, often resulting in increased ionization efficiencies. CovaChem's LCMS Grade Acetic Acid comes in two convenient package sizes (10 x 1 mL Ampules and 25 mL bottles). The 1 mL Ampules of LCMS Grade Acetic Acid are useful for quickly preparing 1 Liter bottles of 0.1% Acetic Acid solutions in either water or other organic solvents

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    Product Information

    Package Sizes: 10 x 1 mL and 25 mL

    Product Numbers: 11201-10x1 or 11201-25

    Product Name: LCMS Grade Acetic Acid

    Alternative Names: LCMS Acetic Acid; Glacial Acetic Acid; Ethanoic Acid; Methanecarboxylic acid


    Specifications

    Appearance: Clear liquid

    Purity: is ≥ 99.8%

    Evaporative Residue: < 8 ppm

    Color Test: 10 APHA

    Identity: Passes Test

    Heavy Metals: ≤ 300 ppb

    Chlorides: ≤ 1.0 ppm

    Sulfates: ≤ 1.0 ppm

    Nickel: ≤ 100 ppb

    Cadmium: ≤ 100 ppb

    Iron (Fe): ≤ 200 ppb

    Absorbance (@350 nm): ≤ 0.02 AU

    Proteases: None Detected

    Reductant Assay: None Detected

    Storage: Room Temperature


    Properties

    Physical State: Liquid

    CAS #: 64-19-7

    Chemical Formula: C2H4O2

    Molecular Weight: 60.05 amu

    pKa: 4.76

    Boiling Point: 118-119 °C (Lit)

    Density: 1.049 g/mL

    Tariff Code: 2915.21.0000


    Additional Product Information:
    LCMS Grade Acetic Acid Product Information Bulletin
    LCMS Grade Acetic Acid Safety Data Sheet SDS
    $32.00
  • LCMS Grade Formic Acid (UltraPure)

    CovaChem's LCMS Grade Formic Acid is ideally suited as a chromatography additive for use in both HPLC and LCMS applications. Formic acid is typically beneficial as a separations enhancer, and has a weak ion pairing effect in the mass spectrometer, making dissociation of from the analytes relatively easy, often resulting in increased ionization efficiencies, while providing the benefit of pH control to the mobile phase. CovaChem's LCMS grade Formic acid is highly refined, and lot tested not only for purity, but also for the absence of metal ions known to be detrimental to mass spectrometer performance and ionization efficiencies.

    CovaChem's LCMS Grade Formic Acid comes in two convenient package sizes (10 x 1 mL Ampules and 25 mL bottles). The 1 mL Ampules of LCMS Grade Formic Acid are particularly useful for quickly preparing 1 Liter bottles of 0.1% Formic Acid solutions in either water or other organic solvents.

    Package Sizes: 10 x 1 mL and 25 mL

    Product Numbers: 11202-10x1 or 11202-25

    Product Name: LCMS Grade Formic Acid

    Alternative Names: LCMS Formic Acid; Formylic Acid; Methanoic Acid; Hydrogencarboxylic acid; Hydroxy(oxo)methane; Aminic acid; Oxomethanol; Oxocarbinic acid


    Specifications

    Appearance: Clear liquid

    Purity: is ≥ 99%

    Storage: Room Temperature


    Properties

    Physical State: Liquid

    CAS #: 64-18-6

    Chemical Formula: CH2O2

    Molecular Weight: 46.03 amu

    pKa: 3.77

    Boiling Point: 100.8 °C (Lit)

    Density: 1.22 g/mL

    Tariff Code: 2915.11.0000

    LCMS Grade Formic Acid Protocol and Product Information
    LCMS Grade Formic Acid MSDS

    $32.00
  • LCMS Grade Formic Acid plus TFA (UltraPure)

    CovaChem's LCMS Grade Formic Acid with TFA from CovaChem is of Ultra High Purity ideally suited for sensitive LCMS and HPLC applications. This mixture offers the ability to quickly and easily make one liter of 0.1% Formic Acid with 0.01% TFA enabling the exceptional resolving powers of Trifluoroacetic acid (TFA) without reducing ionization efficiencies.

    LCMS Grade Formic Acid is ideally suited as a chromatography additive for use in LCMS applications, where the ion pairing effects of TFA is needed, but high Mass Spectrometry sensitivity is also required.

    CovaChem's LCMS Grade Formic Acid with TFA comes in two convenient package sizes (10 x 1.1 mL Ampules and 25 mL bottles). The 1.1 mL Ampules of LCMS Grade Formic Acid are useful for quickly preparing 1 Liter bottles of 0.1% Formic Acid with 0.01% TFA solutions in either water or other organic solvents.

    Package Sizes: 10 x 1.1 mL and 25 mL
    Product Numbers: 11207-10x1 or 11207-25

    Product Name: LCMS Grade Formic Acid with TFA
    Alternative Names: Mixture of Formic Acid and TFA, TFA fix, Formic Acid + TFA

    $42.00
  • LCMS Grade HFBA (UltraPure)

    CovaChem's LCMS Heptafluorbutyric acid is well suited as a chromatography additive for use in HPLC and LCMS applications. Heptafluorobutyric acid (HFBA) is typically beneficial as a separations enhancer, and is a moderately strong ion pairing agent, which can be a very effective way of gaining good analyte separation and sharp peaks in HPLC applications. This moderately strong ion pairing effect can unfortunately reduce ionization efficiencies (compared to other acid additives, such as Formic acid (11202) and Acetic acid (11201). This reduction of ionization efficiency can often be overcome by the incorporation of the post-column addition a “TFA fix” reagent, such as a highly refined (2-(2-methoxyethoxy) ethanol (CovaChem 11207) or LCMS Grade Propionic acid (CovaChem 11207). Please feel free to contact our technical support for more information on this subject.

    CovaChem's LCMS Grade HFBA comes in two convenient package sizes (10 x 1 mL Ampules and 25 mL bottles). The 1 mL Ampules of LCMS Grade HFBA are useful for quickly preparing 1 Liter bottles of 0.1% HFBA solutions in either water or other organic solvents.

    Package Sizes: 10 x 1 mL and 25 mL
    Product Numbers: 11203-10x1 or 11203-25

    Product Name: LCMS Grade HFBA
    Alternative Names: LCMS HFBA; LCMS HFBA Amps; Heptafluorobutyric acid Ampules; Perfluorobutanoic acid; CAS# 375-22-4; LCMS Heptafluorobutyric acid; Perfluoricbutyric acid; 2,2,3,3,4,4,4-Heptafluorobutanoic acid

    Specifications
    Appearance: Clear liquid
    Purity: is ≥ 99%
    Storage: Room Temperature

    Properties
    Physical State: Liquid
    CAS #: 375-22-4
    Chemical Formula: C4HF7O2
    Molecular Weight: 214.04 amu
    pKa: 3.77
    Boiling Point: 120 °C (Lit)
    Density: 1.645 g/mL
    Tariff Code: 2915.90.1800
    $43.00
  • LCMS Grade Propionic acid (UltraPure)

    CovaChem's LCMS Grade Propionic acid (Ultra High Purity) from CovaChem is well suited for HPLC and LCMS applications. Propionic acid can be used as a mobile phase for HPLC or LCMS, or can be used post-column in LCMS applications to increase ionization efficiencies when ion pairing agents are used for separation.

    CovaChem's LCMS Grade Propionic acid (Ultra High Purity) comes in two convenient package sizes (10 x 1 mL Ampules and 25 mL bottles). The 1 mL Ampules of LCMS Grade Propionic acid are useful for quickly preparing 1 Liter bottles of 0.1% Propionic acid solutions in either water or other organic solvents.

    Package Sizes: 10 x 1 mL and 25 mL (Chose size below from dropdown menu).
    Product Numbers: 11206-10x1 or 11206-25

    Product Name: LCMS Grade Propionic acid
    Alternative Names: Ethanecarboxylic acid; Propionic acid; Propanyl acid; CAS# 79-09-4

    Specifications
    Appearance: Clear liquid
    Purity: is ≥ 99.5%
    Storage: Room Temperature

    Properties
    Physical State: Liquid
    CAS #: 79-09-4
    Chemical Formula: C3H6O2
    Molecular Weight: 74.08 amu
    pKa: 4.87
    Boiling Point: 141 °C (Lit)
    Density: 0.99 g/mL
    Tariff Code: 2915.50.1100

    $39.00
  • Triethylamine (UltraPure)

    CovaChem's LCMS Grade Triethylamine (TEA) is well suited as a chromatography additive for difficult to separate analytes in HPLC and LCMS applications. Triethylamine (TEA) is a useful ion pairing agent, that is often necessary for optimal separation in HPLC and LCMS applications.

    Triethylamine is an ion pairing agent that is often used in conjugation with other volatile acids creating close to neutral buffers.

    CovaChem's LCMS Grade Triethylamine (TEA) comes in two convenient package sizes (10 x 1 mL Ampules and 25 mL bottles). The 1 mL Ampules of LCMS Grade TEA are useful for quickly preparing 1 Liter bottles of 0.1% TEA solutions in either water or other organic solvents.

    Package Sizes: 10 x 1 mL and 25 mL (Chose size below from dropdown menu).
    Product Numbers: 11205-10x1 or 11205-25

    Product Name: LCMS Grade TEA
    Alternative Names: LCMS TEA; LCMS TEA Amps; LCMS Grade TEA Amps; Triethylamine; N,N-Diethylethanamine; N,N,N-Triethylamine; (Diethylamino)ethane; CAS# 121-44-8

    Specifications
    Appearance: Clear liquid
    Purity: is ≥ 99.5%
    Storage: Room Temperature

    Properties
    Physical State: Liquid
    CAS #: 121-44-8
    Chemical Formula: C6H15N
    Molecular Weight: 101.19 amu
    pKa: 10.65
    Boiling Point: 89-90 °C (Lit)
    Density: 0.7255 g/mL
    Tariff Code: 2921.19.1100
    $41.00
  • Trifluoroacetic Acid (LCMS Grade TFA)

    Product Name: LCMS Grade TFA (Trifluoroacetic acid)

    Alternative Names: LCMS TFA; LCMS TFA Amps; TFA Ampules; Trifluoroacetic acid; TFA Acid; CAS# 76-05-1; Perfluoric acid; 2,2,2-Trifluoroacetic acid; HPLC TFA


    CovaChem's LCMS Grade Trifluoroacetic acid (TFA) is well suited as a chromatography additive for difficult to separate analytes in HPLC and LCMS applications. CovaChem's LCMS TFA is an ideal additive for separations of peptides in peptide synthesis applications. Trifluoroacetic acid is widely used to separate both protected amino acid chains and deprotected peptides. Due to the amino and carboxylate groups having vastly different pka values, synthetic peptides can be particularly challenging to separate, The HPLC separation of peptides is much more easily achieved with 0.1% Trifluoroacetic acid being present in the mobile phase.

    Trifluoroacetic acid (TFA) is typically beneficial as a separations enhancer, and is a moderately strong ion pairing agent, which can be a very effective way of gaining good analyte separation and obtaining sharp peaks in HPLC applications. Although TFA is and excellent low pH buffer for HPLC applications, this ion pairing effect has some negative impact on ionization efficiencies (compared to other acid additives, such as Formic acid (CovaChem 11202) and Acetic acid (CovaChem 11201. CovaChem offers several remedies for the impact of 0.1% TFA on ionization, in serving highly refined products that reverse this effect. This reduction of ionization efficiency can be overcome by incorporating a post-column addition of a “TFA fix” reagent, such as CovaChem's UltraPure (2-(2-methoxyethoxy) ethanol (CovaChem 11207) or LCMS Grade Propionic acid (CovaChem 11206). These products are easy to employ, and allow LCMS to be performed on peptides and proteins without altering HPLC methods that employ 0.1% TFA. These tfa fix reagents have saved countless hours of hplc method development and validation hours. Please feel free to contact our technical support for more information on this subject.

    CovaChem's LCMS Grade TFA comes in two convenient package sizes (10 x 1 mL Ampules and 25 mL bottles). The 1 mL Ampules of LCMS Grade TFA are useful for quickly preparing 1 Liter bottles of 0.1% TFA solutions in either water or other organic solvents.

    Trifluoroacetic acid (CAS# 76-05-1) ampules for LCMS

    Package Sizes: 10 x 1 mL and 25 mL
    Product Numbers: 11204-10x1 or 11204-25

    Specifications
    Appearance: Clear liquid
    Purity: is ≥ 99.5%
    Storage: Room Temperature

    Properties
    Physical State: Liquid
    CAS #: 76-05-1
    Chemical Formula: C2HF2O2
    Molecular Weight: 114.02 amu
    pKa: 0.23
    Boiling Point: 72.4 °C (Lit)
    Density: 1.489 g/mL
    Tariff Code: 2915.90.5000

    Additional Product Information:
    Trifluoroacetic Acid (TFA) Product Information Bulletin
    Trifluoroacetic Acid (TFA) MSDS

    $35.00