The useful application of Beta-Glucuronidase in hydrolysis of glucuronides. Organic mechanism of glucuronide conjugate hydrolytic cleavage.

This article describes the reaction of Sulfo-NHS esters with proteins and peptides in buffered reaction systems. Several of the key reaction concerns are covered here. This post is intended to provide some background for protein research scientists before planning experiments involving this class of compounds.

This article describes some important aspects of selecting the best mobile phase for a specific application. Posting also covers some of the more common mobile phase additives used in LC/MS.

This article describes some of the useful features of the homobifunctional crosslinker 3,3'-Dithiobis (sulfosuccinimidyl propionate), also known as DTSSP.

MALDI is a commonly employed technique for analyzing proteins and other biomolecules by mass spectrometry. This soft ionization technique can be ideal for deciphering the mass and composition of proteins and peptide fragments, but often require some forethought into experimental design.