Trifluoroacetic Acid (LCMS Grade TFA)

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Trifluoroacetic Acid (LCMS Grade TFA)
Package Size: 10 x 1 mL Ampules
SKU: 11204-10x1
WEIGHT: 0.20
Trifluoroacetic Acid (LCMS Grade TFA)
Package Size: 25 mL Bottle
SKU: 11204-25
WEIGHT: 0.20
Trifluoroacetic Acid (LCMS Grade TFA)
Package Size: 4 x 25 mL Bottles
SKU: 11204-4x25
WEIGHT: 0.20

Product Name: LCMS Grade TFA (Trifluoroacetic acid)

Alternative Names: LCMS TFA; LCMS TFA Amps; TFA Ampules; Trifluoroacetic acid; TFA Acid; CAS# 76-05-1; Perfluoric acid; 2,2,2-Trifluoroacetic acid; HPLC TFA

CovaChem's LCMS Grade Trifluoroacetic acid (TFA) is well suited as a chromatography additive for difficult to separate analytes in HPLC and LCMS applications. CovaChem's LCMS TFA is an ideal additive for separations of peptides in peptide synthesis applications. Trifluoroacetic acid is widely used to separate both protected amino acid chains and deprotected peptides. Due to the amino and carboxylate groups having vastly different pka values, synthetic peptides can be particularly challenging to separate, The HPLC separation of peptides is much more easily achieved with 0.1% Trifluoroacetic acid being present in the mobile phase.

Trifluoroacetic acid (TFA) is typically beneficial as a separations enhancer, and is a moderately strong ion pairing agent, which can be a very effective way of gaining good analyte separation and obtaining sharp peaks in HPLC applications. Although TFA is and excellent low pH buffer for HPLC applications, this ion pairing effect has some negative impact on ionization efficiencies (compared to other acid additives, such as Formic acid (CovaChem 11202) and Acetic acid (CovaChem 11201. CovaChem offers several remedies for the impact of 0.1% TFA on ionization, in serving highly refined products that reverse this effect. This reduction of ionization efficiency can be overcome by incorporating a post-column addition of a “TFA fix” reagent, such as CovaChem's UltraPure (2-(2-methoxyethoxy) ethanol (CovaChem 11207) or LCMS Grade Propionic acid (CovaChem 11206). These products are easy to employ, and allow LCMS to be performed on peptides and proteins without altering HPLC methods that employ 0.1% TFA. These tfa fix reagents have saved countless hours of hplc method development and validation hours. Please feel free to contact our technical support for more information on this subject.

CovaChem's LCMS Grade TFA comes in two convenient package sizes (10 x 1 mL Ampules and 25 mL bottles). The 1 mL Ampules of LCMS Grade TFA are useful for quickly preparing 1 Liter bottles of 0.1% TFA solutions in either water or other organic solvents.

Trifluoroacetic acid (CAS# 76-05-1) ampules for LCMS

Package Sizes: 10 x 1 mL and 25 mL
Product Numbers: 11204-10x1 or 11204-25

Appearance: Clear liquid
Purity: is ≥ 99.5%
Storage: Room Temperature

Physical State: Liquid
CAS #: 76-05-1
Chemical Formula: C2HF2O2
Molecular Weight: 114.02 amu
pKa: 0.23
Boiling Point: 72.4 °C (Lit)
Density: 1.489 g/mL
Tariff Code: 2915.90.5000

Additional Product Information:
Trifluoroacetic Acid (TFA) Product Information Bulletin
Trifluoroacetic Acid (TFA) MSDS

  • Biotin-LC Hydrazide

    Biotin-LC Hydrazide

    Long chain biotinylation reagent for carbohydrates. Biotin-LC-Hydrazide
  • Biotin-NHS  (CAS 35013-72-0)

    Biotin-NHS (CAS 35013-72-0)

    NHS-Biotin is a simple, amine reactive biotinylation reagent.
  • BMPS Crosslinker

    BMPS Crosslinker

    3-Maleimidopropionic acid N-hydroxysuccinimide ester (BMPS) is a heterobifunctional crosslinker with a spacer length of 6.9 Å. BMPS contains an amine reactive, NHS ester, and a maleimide group which is reactive towards sulfhydryl functional groups.
  • DCC (Dicyclohexylcarbodiimide)

    DCC (Dicyclohexylcarbodiimide)

    N,N''-Dicyclohexylcarbodiimide (DCC) is a zero length crosslinker. Dicyclohexylcarbodiimide is a coupling reagent used to couple carboxylic acids (COOH) to primary amines (-NH2).
  • DMSO (Dimethylsulfoxide)

    DMSO (Dimethylsulfoxide)

    CovaChem's Molecular Biology Grade DMSO is a high purity solvent intended for use in protein assays, chromatography, and other highly sensitive applications.
  • HPLC Grade Isopropanol

    HPLC Grade Isopropanol

    CovaChem's HPLC Grade Isopropanol (2-Propanol) is a high purity solvent well suited for HPLC applications, chemical synthesis and general laboratory use.
  • Iodoacetamide (IAA)

    Iodoacetamide (IAA)

    Iodoacetamide (IAA)
  • LC-SPDP Crosslinker

    LC-SPDP Crosslinker

    Succinimidyl 6-[3-(2-pyridyldithio)propionamido] hexanoate (LC-SPDP) is a heterobifunctional crosslinker that is amino reactive on one end and sulfhydryl reactive on the other end. LC-SPDP contains a long spacer arm (15.7 Å)for increased molecular flexibility and a reducible disulfide, which is allows for cleavage of the crosslinker after crosslink formation.
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