Trifluoroacetic Acid (LCMS Grade TFA)

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Trifluoroacetic Acid (LCMS Grade TFA)
Package Size: 10 x 1 mL Ampules
SKU: 11204-10x1
WEIGHT: 0.20
Trifluoroacetic Acid (LCMS Grade TFA)
Package Size: 25 mL Bottle
SKU: 11204-25
WEIGHT: 0.20
Trifluoroacetic Acid (LCMS Grade TFA)
Package Size: 4 x 25 mL Bottles
SKU: 11204-4x25
WEIGHT: 0.20

Product Name: LCMS Grade TFA (Trifluoroacetic acid)

Alternative Names: LCMS TFA; LCMS TFA Amps; TFA Ampules; Trifluoroacetic acid; TFA Acid; CAS# 76-05-1; Perfluoric acid; 2,2,2-Trifluoroacetic acid; HPLC TFA

CovaChem's LCMS Grade Trifluoroacetic acid (TFA) is well suited as a chromatography additive for difficult to separate analytes in HPLC and LCMS applications. CovaChem's LCMS TFA is an ideal additive for separations of peptides in peptide synthesis applications. Trifluoroacetic acid is widely used to separate both protected amino acid chains and deprotected peptides. Due to the amino and carboxylate groups having vastly different pka values, synthetic peptides can be particularly challenging to separate, The HPLC separation of peptides is much more easily achieved with 0.1% Trifluoroacetic acid being present in the mobile phase.

Trifluoroacetic acid (TFA) is typically beneficial as a separations enhancer, and is a moderately strong ion pairing agent, which can be a very effective way of gaining good analyte separation and obtaining sharp peaks in HPLC applications. Although TFA is and excellent low pH buffer for HPLC applications, this ion pairing effect has some negative impact on ionization efficiencies (compared to other acid additives, such as Formic acid (CovaChem 11202) and Acetic acid (CovaChem 11201. CovaChem offers several remedies for the impact of 0.1% TFA on ionization, in serving highly refined products that reverse this effect. This reduction of ionization efficiency can be overcome by incorporating a post-column addition of a “TFA fix” reagent, such as CovaChem's UltraPure (2-(2-methoxyethoxy) ethanol (CovaChem 11207) or LCMS Grade Propionic acid (CovaChem 11206). These products are easy to employ, and allow LCMS to be performed on peptides and proteins without altering HPLC methods that employ 0.1% TFA. These tfa fix reagents have saved countless hours of hplc method development and validation hours. Please feel free to contact our technical support for more information on this subject.

CovaChem's LCMS Grade TFA comes in two convenient package sizes (10 x 1 mL Ampules and 25 mL bottles). The 1 mL Ampules of LCMS Grade TFA are useful for quickly preparing 1 Liter bottles of 0.1% TFA solutions in either water or other organic solvents.

Trifluoroacetic acid (CAS# 76-05-1) ampules for LCMS

Package Sizes: 10 x 1 mL and 25 mL
Product Numbers: 11204-10x1 or 11204-25

Appearance: Clear liquid
Purity: is ≥ 99.5%
Storage: Room Temperature

Physical State: Liquid
CAS #: 76-05-1
Chemical Formula: C2HF2O2
Molecular Weight: 114.02 amu
pKa: 0.23
Boiling Point: 72.4 °C (Lit)
Density: 1.489 g/mL
Tariff Code: 2915.90.5000

Additional Product Information:
Trifluoroacetic Acid (TFA) Product Information Bulletin
Trifluoroacetic Acid (TFA) MSDS

  • 4-carbethoxyhexafluorobutyrl chloride (4-CB)

    4-carbethoxyhexafluorobutyrl chloride (4-CB) is an alkylation reagent used in GC derivatization for improved gas chromatographic separations.
  • CHCA MALDI Matrix

    CHCA MALDI Matrix is a reagent used to create ions for MALDI-MS and MALDI TOF TOF analysis of protein and peptide molecules below 4,000 Daltons. TOF TOF mass spectrometry is one of the more common forms of MALDI-MS/MS, although several other types of mass spectrometers can also be coupled to MALDI instrumentation.
  • DMSO (Dimethylsulfoxide)

    CovaChem's Molecular Biology Grade DMSO is a high purity solvent intended for use in protein assays, chromatography, and other highly sensitive applications.
  • DTSSP Crosslinker

    3,3'-Dithiobis(sulfosuccinimidyl propionate), commonly known as DTSSP, is a reducible amine reactive protein crosslinking reagent. DTSSP is water soluble, and does not permeate the cell membrane.
  • HPLC Grade Acetone

    CovaChem's HPLC Grade Acetone is a high purity solvent ideally suited for HPLC applications and general laboratory use.
  • LCMS Grade Acetic Acid (UltraPure)

    LCMS Grade Acetic Acid (CAS# 64-19-7) from CovaChem is of Ultra High Purity ideally suited for sensitive LCMS and HPLC applications.
  • LCMS Grade Acetonitrile

    CovaChem's LCMS Grade Acetonitrile is a high purity solvent ideally suited for LC/MS and other mass spectrometry related applications.
  • LCMS Grade Formic Acid plus TFA (UltraPure)

    LCMS Grade Formic Acid with TFA from CovaChem is of Ultra High Purity ideally suited for sensitive LCMS and HPLC applications. This mixture offers the ability to quickly and easily make one liter of 0.1% Formic Acid with 0.01% TFA enabling the exceptional resolving powers of Trifluoroacetic acid (TFA) without reducing ionization efficiencies.
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