Trifluoroacetic Acid (LCMS Grade TFA)

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Trifluoroacetic Acid (LCMS Grade TFA)
Package Size: 10 x 1 mL Ampules
SKU: 11204-10x1
WEIGHT: 0.20
$35.00
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Trifluoroacetic Acid (LCMS Grade TFA)
Package Size: 25 mL Bottle
SKU: 11204-25
WEIGHT: 0.20
$49.00
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Trifluoroacetic Acid (LCMS Grade TFA)
Package Size: 4 x 25 mL Bottles
SKU: 11204-4x25
WEIGHT: 0.20
$119.00
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Product Name: LCMS Grade TFA (Trifluoroacetic acid)

Alternative Names: LCMS TFA; LCMS TFA Amps; TFA Ampules; Trifluoroacetic acid; TFA Acid; CAS# 76-05-1; Perfluoric acid; 2,2,2-Trifluoroacetic acid; HPLC TFA


CovaChem's LCMS Grade Trifluoroacetic acid (TFA) is well suited as a chromatography additive for difficult to separate analytes in HPLC and LCMS applications. CovaChem's LCMS TFA is an ideal additive for separations of peptides in peptide synthesis applications. Trifluoroacetic acid is widely used to separate both protected amino acid chains and deprotected peptides. Due to the amino and carboxylate groups having vastly different pka values, synthetic peptides can be particularly challenging to separate, The HPLC separation of peptides is much more easily achieved with 0.1% Trifluoroacetic acid being present in the mobile phase.

Trifluoroacetic acid (TFA) is typically beneficial as a separations enhancer, and is a moderately strong ion pairing agent, which can be a very effective way of gaining good analyte separation and obtaining sharp peaks in HPLC applications. Although TFA is and excellent low pH buffer for HPLC applications, this ion pairing effect has some negative impact on ionization efficiencies (compared to other acid additives, such as Formic acid (CovaChem 11202) and Acetic acid (CovaChem 11201. CovaChem offers several remedies for the impact of 0.1% TFA on ionization, in serving highly refined products that reverse this effect. This reduction of ionization efficiency can be overcome by incorporating a post-column addition of a “TFA fix” reagent, such as CovaChem's UltraPure (2-(2-methoxyethoxy) ethanol (CovaChem 11207) or LCMS Grade Propionic acid (CovaChem 11206). These products are easy to employ, and allow LCMS to be performed on peptides and proteins without altering HPLC methods that employ 0.1% TFA. These tfa fix reagents have saved countless hours of hplc method development and validation hours. Please feel free to contact our technical support for more information on this subject.

CovaChem's LCMS Grade TFA comes in two convenient package sizes (10 x 1 mL Ampules and 25 mL bottles). The 1 mL Ampules of LCMS Grade TFA are useful for quickly preparing 1 Liter bottles of 0.1% TFA solutions in either water or other organic solvents.

Trifluoroacetic acid (CAS# 76-05-1) ampules for LCMS

Package Sizes: 10 x 1 mL and 25 mL
Product Numbers: 11204-10x1 or 11204-25

Specifications
Appearance: Clear liquid
Purity: is ≥ 99.5%
Storage: Room Temperature

Properties
Physical State: Liquid
CAS #: 76-05-1
Chemical Formula: C2HF2O2
Molecular Weight: 114.02 amu
pKa: 0.23
Boiling Point: 72.4 °C (Lit)
Density: 1.489 g/mL
Tariff Code: 2915.90.5000

Additional Product Information:
Trifluoroacetic Acid (TFA) Product Information Bulletin
Trifluoroacetic Acid (TFA) MSDS

  • 2-Iminothiolane

    2-Iminothiolane

    2-Iminothiolane hydrochloride (Traut's Reagent) is used to convert primary amine (-NH2) groups to sulfhydryl groups (-SH) at pH 7 to 10. Traut's reagent (2-IT) is a water soluble molecule that is easy to use and creates a wide range of flexibility to the researcher.
    $69.00
  • D-Biotin

    D-Biotin

    Biotin is a compound used to biotinylate proteins with the addition of a zero length cross linking reagent. D-Biotin has several biology research applications, where the avidin biotin interaction is utilized.
    $27.00
  • EGS Crosslinker

    EGS Crosslinker

    Ethylene glycol bis(succininic acid) N-hydroxysuccinimide ester) is a cleavable, homobifuntional crosslinker that contains two NHS functional groups. The NHS functional groups of EGS are selectively reactive towards primary amines at pH 7 to 9. EGS is cleavable with hydroxylamine at pH 8.5. EGS is often used as a cleavable option when disulfide containing crosslinkers, such as DSP (CovaChem 13304) and DTSSP (CovaChem 13303) are not an option due to the desire to retain disulfide networks of a protein intact.
    $39.00
  • HPLC Grade Acetonitrile

    HPLC Grade Acetonitrile

    CovaChem's HPLC Grade Acetonitrile is a high purity solvent ideally suited for HPLC applications and general laboratory use.
    $87.00
  • Iodoacetamide (IAA)

    Iodoacetamide (IAA)

    Iodoacetamide (IAA)
    $49.00
  • LCMS Grade Formic Acid (UltraPure)

    LCMS Grade Formic Acid (UltraPure)

    LCMS Grade Formic Acid (CAS# 64-18-6) from CovaChem is of Ultra High Purity ideally suited for sensitive LCMS and HPLC applications.
    $32.00
  • LCMS Grade HFBA (UltraPure)

    LCMS Grade HFBA (UltraPure)

    LCMS Grade Formic Acid (CAS# 375-22-4) from CovaChem is of very high purity and well suited for HPLC and LCMS applications, where separations are difficult otherwise.
    $43.00
  • Molecular Biology Grade HEPES

    Molecular Biology Grade HEPES

    HEPES is well suited as a buffer for many protein and cellular assays.
    $18.00
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