Homobifunctional Crosslinkers


Protein Crosslinkers-Homobifunctional Homobifunctional crosslinkers have two reactive groups that are identical in structure and have the same reaction specificity. NHS and Sulfo-NHS homobifunctional compounds are some of the more common homobifunctional protein crosslinkers available.

Crosslinking with homobifunctional crosslinkers is an ideal strategy for locking two proteins in place with a covalent bond. These crosslinked proteins can be analyzed by numerous techniques. Typically, covalent bonds formed by chemical crosslinkers are resistant to protease cleavage, allowing subsequent enzymatic digestion after protein crosslinking. This technique is commonly utilized for protein mapping experiments, where mass spectrometry is performed to detect interacting peptide regions between two proteins or a folding region of a single protein.

Please inquire with technical support for any homobifunctional crosslinkers of interest that may not be listed on CovaChem.com.

  • BS2G Crosslinker

    Bis-(sulfosuccinimidyl) glutarate (BS2G) is soluble in aqueous solutions, which enhances its ease of use, as organic solvents can be avoided entirely. Sulfo-DSG (BS2G) is membrane impermeable, which makes it an ideal candidate for membrane protein crosslinking. BS2G contains two sulfo-NHS groups which react selectively with amine groups at pH 7 to 9.

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 13302-100 and 13302-1

    Product Name: BS2G Crosslinker

    Alternative Names: BS2G; Sulfo-DSG; Bis-(sulfosuccinimidyl) glutarate; Sulfo-DSG crosslinker; Sulfosuccinimidyl glutarate; Di(N-sulfosuccinimidyl) glutarate; sodium 1,1'-(glutaroylbis(oxy))bis(2,5-dioxopyrrolidine-3-sulfonate); BS2G-d0; C13H12N2 Na2 O14 S2

    Specifications

    Appearance: White Solid

    Purity: is ≥ 90% by quantitative NMR

    Storage: 4 °C, protected from moisture

     

    Properties Physical State: Solid

    CAS#: N/A

    Chemical Formula: C13H12N2 Na2 O14 S2

    Molecular Weight: 530.35

    Spacer length: 7.7 Å

    Country of Origin: USA

    Tariff Code: 2925.19.9100

    $89.00
  • BS3 Crosslinker

    Bis-sulfosuccinimidyl suberate (BS3) is a water soluble, non-cleavable, membrane impermeable homobifunctional protein crosslinker. BS3 is homobifunctional protein crosslinker with targeted reactivity towards primary amines at pH 7 to pH 9. Proteins are often a rich source of amines, both in lysine (K) residues and the N-terminus. Once reacted with the amine (-NH2) group, amide bond is formed. 

    Package Sizes: 10 x 5 mg, 100 mg and 1 gram

    Product Numbers: 13306-10x5, 13306-100 and 13306-1

    Product Name: BS3 Crosslinker

    Alternative Names: bis-sulfosuccinimidyl suberate; BS3; sulfo-DSS; bis-sulfo-succinimidyl suberate; 82436-77-9; C16H18N2Na2O14S2; bissulfosuccinimidyl suberate; Sulfo-DSS; Suberic acid sulfo-NHS ester; BSSS; BS3-d0; sodium 1,1'-(octanedioylbis(oxy))bis(2,5-dioxopyrrolidine-3-sulfonate), BS3 protein crosslinker

    Specifications

    Appearance: White Solid

    Purity: is ≥ 90% Storage: 4 °C, protected from moisture

     

    Properties Physical State: Solid

    CAS#: 82436-77-9

    Chemical Formula: C16H18N2Na2O14S2

    Molecular Weight: 572.43

    Spacer length: 11.4 Å

    Melting Point: >300 °C (Lit)

    Country of Origin: USA

    Tariff Code: 2925.19.9100


    Additional Product Information:
    BS3 Product Information Bulletin
    BS3 Crosslinker MSDS
    $89.00
  • DSG Crosslinker

    Disuccinimidyl glutarate (DSG) is a cell membrane permeable, homobifunctional crosslinker with a five carbon spacer arm. DSG contains two NHS esters that are ideal for selectively targeting primary amine moieties at pH 7 to 9. DSG is a simple, short length crosslinker that forms irreversible amide bonds with lysine residues. This compound is ideally suited for a wide range of crosslinking applications, where a cleavable crosslinker is not preferential.

    This compound is also available in a water soluble form (BS2G), which enables direct addition to aqueous buffers, when the user prefers not to use any organic solvents in their crosslinking applications.

    CovaChem's DSG is among the highest purity in the industry, with an absolute purity specification of ≥ 97%, measured with quantitative NMR against an internal standard, and typical batches have purity values close to 99% pure.

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 13301-100 and 13301-1

    Product Name: DSG Crosslinker

    Alternative Names: DSG, discuccinimidyl glutarate, DSG crosslinker, Succinimidyl glutarate, Di(N-succinimidyl) glutarate, 2,5-dioxopyrrolidin-1-yl 6-(2,5-dioxopyrrolidin-1-yl)-5-oxohexanoate; C13H14N2 O8

    Specifications

    Appearance: White Solid

    Purity: is ≥ 97% by quantitative NMR

    Storage: 4 °C, protected from moisture

    Properties

    Properties Physical State: Solid

    CAS#: 79642-50-5

    Chemical Formula: C13H14N2 O8

    Molecular Weight: 326.26

    Spacer length: 7.7 Å

    Melting Point: 142-145 °C (Lit)

    Country of Origin: USA

    Tariff Code: 2928.00.5000


    Additional Product Information:
    DSG Product Information Bulletin
    DSG Crosslinker MSDS
    $32.00
  • DSP Crosslinker

    DSP (DTSP) is a reducible crosslinking reagent. DSP is membrane permeable, homobifunctional crosslinker that is reactive towards amine (amino) functional groups. After reaction with proteins or peptides, the disulfide containing crosslinked protein can be reduced with 2-mercaptoethanol (CovaChem 11301-10x1).

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 13303-100 and 13303-1

    Product Name: DSP Crosslinker

    Alternative Names: Lomant's Reagent; Dithiobis(succinimidyl propionate); DTSP; DSP; DSP-d0; 79642-50-5; C14H16N2 O8S2

    Specifications

    Appearance: White Solid

    Purity: is ≥ 92% Storage: 4 °C, protected from moisture

     

    Properties Physical State: Solid

    CAS#: 57757-57-0

    Chemical Formula: C14H16N2 O8S2

    Molecular Weight: 404.42

    Spacer length: 12.0 Å

    Melting Point: 128-132 °C (Lit)

    Country of Origin: USA

    Tariff Code: 2930.90.9190

    $39.00
  • DSS Crosslinker

    Disuccinimidyl Suberate (DSS) is a homobifunctional crosslinker that reacts with primary amines.
    $32.00
  • DST Crosslinker

    Disuccinimidyl tartrate (DST) contains two NHS esters which are reactive towards amino (amine) groups. DST is cleavable with oxidizing agents, such as sodium periodate. This crosslinker allows researchers another option for crosslinker cleavage, without degrading S-S (disulfide) linkages.

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 13407-100 and 13407-1

    Product Name: DST Crosslinker

    Alternative Names: DST; Disuccinimidyl tartrate; 62069-75-4; Disuccinimidyl-L-tartrate; Disuccinimidyl tartarate; C12H12N2O10

    Specifications

    Appearance: White to Pale Yellow Solid

    Purity: is ≥ 90% by quantitative NMR

    Storage: 4 °C, protected from moisture

     

    Properties Physical State: Solid

    CAS#: 62069-75-4

    Chemical Formula: C12H12N2O10

    Molecular Weight: 344.23

    Spacer length: 6.4 Å

    Melting Point: 168 °C (Lit)

    Country of Origin: USA


    Additional Product Information:
    DST Product Information Bulletin
    DST Crosslinker SDS

    $119.00
  • DTSSP Crosslinker

    DTSSP is a cleavable protein crosslinking reagent that contains a disulfide moeity in its eight atom spacer arm. DTSSP is a membrane impermeable, homobifunctional crosslinker that is reactive towards amine (amino) functional groups. In the pH range of 7-9, this reaction is particularly selective for primary amine groups, resulting in the formation of stable amide linkages. Phosphate buffers and phosphate buffered saline are typically used for crosslinking reactions involving DTSSP, although others non-amine containing buffers may also be used.

    Once the reaction between DTSSP and proteins or peptides is complete, the disulfide-containing crosslinked protein can be cleaved via reduction of the crosslinkers disulfide bond using a reducing agent, such as 2-mercaptoethanol (CovaChem 11301-10x1) or DTT (CovaChem 11302-10x5). CovaChem's technical support team is readily available to assist with any individual optimization.

    Dithiobis(sulfosuccinimidyl propionate) (DTSSP) is the water soluble version of Lomant's Reagent (DSP). Both DSP and DTSSP have been used extensively in protein crosslinker applications as well as other scientific applications involving forming a stable linkages between the disulfide group and metal surfaces, while leaving the NHS functional groups available to react with other nucleophiles. This application can be useful for immobilizing proteins or other molecules to the surface of gold beads or other foil.

    Due to the lipophobic nature of the sulfonate groups on DTSSP, this crosslinker does not permeate the cell membrane. This feature makes DTSSP particularly useful for crosslinking membrane proteins on the surface of the cell without interfering with intercellular proteins.

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 13304-100 and 13304-1

    Product Name: DTSSP Crosslinker

    Alternative Names: DTSSP; 3,3'-Dithiobis(sulfosuccinimidyl propionate); Dithiobis(sulfosuccinimidyl propionate); 81069-02-5; cleavable Sulfo-NHS crosslinker; DTSSP Protein Crosslinker

    Specifications

    Appearance: White Solid

    Purity: is ≥ 90% Storage: 4 °C, protected from moisture

    Properties

    Properties Physical State: Solid

    CAS#: 81069-02-5

    Chemical Formula: C14H14N2Na2O14S4

    Molecular Weight: 608.51

    Spacer length: 12.0 Å

    Additional Product Information:

    DTSSP Product Information Bulletin
    DTSSP Crosslinker MSDS

    $158.00
  • EGS Crosslinker

    Ethylene glycol bis(succininic acid) N-hydroxysuccinimide ester) is a cleavable, homobifuntional crosslinker that contains two NHS functional groups. The NHS functional groups of EGS are selectively reactive towards primary amines at pH 7 to 9. After crosslinking, the EGS spacer arm is cleavable with Hydroxylamine (CovaChem 11304) at pH 8.5. EGS is often used as a cleavable option when disulfide containing crosslinkers, such as DSP (CovaChem 13304 ) and DTSSP (CovaChem 13303) are not an option due to the desire to retain disulfide networks of a protein intact.

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 13408-100 and 13408-1

    Product Name: EGS Crosslinker

    Alternative Names: EGS; ethylene glycol bis(succinimidylsuccinate); ethylene glycol di(succinimidylsuccinate); ethylene glycoldisuccinate di(N-succinimidyl) ester; 70539-42-3; Di(N-succinimidyl) ethylene glycol disccuinate; C18H20N2O12

    Specifications

    Appearance: White Solid

    Purity: is ≥ 90% by quantitative NMR

    Storage: 4 °C, protected from moisture

     

    Properties Physical State: Solid

    CAS#: 70539-42-3

    Chemical Formula: C18H20N2O12

    Molecular Weight: 456.36

    Spacer length: 16.1 Å

    Country of Origin: USA

    $39.00
  • Sulfo-EGS Crosslinker

    Ethylene glycol bis(sulfosuccinimidylsuccinate) (Sulfo-EGS) is a water soluble, cleavable, homobifunctional crosslinker that is reactive towards amine (-NH2) functional groups. Sulfo-EGS crosslinker creates both intramolecular and intermolecular crosslinks between lysine moieties of proteins that are easily cleavable by treatment Hydroxylamine-HCl (CovaChem 11304-25).

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 13309-100 and 13309-1

    Product Name: Sulfo-EGS Crosslinker

    Alternative Names: Ethylene glycol bis(sulfosuccinimidylsuccinate); Bis(sulfo-N-succinimidyl) ethylene glycol disuccinate sodium salt, Ethylene glycol bis(sulfosuccinimidyl succinate) sodium salt, Sulfo EGS; CAS# 142702-32-7;

    Specifications

    Appearance: White Solid

    Purity: is ≥ 90%

    Storage: 4 °C, protected from moisture

    Properties

    Properties Physical State: Solid

    CAS#: 167410-92-6

    Chemical Formula: C18H18N2Na2O18S2

    Molecular Weight: 660.45

    Spacer length: 16.1 Å

    International Shipping

    Tariff Code: 2925.19.9100


    Additional Product Information:
    Sulfo-EGS Product Information Bulletin
    Sulfo-EGS Crosslinker MSDS

    $109.00