Heterobifunctional Crosslinkers


Protein Crosslinkers-Heterobifunctional

Heterobifunctional protein crosslinkers contain two different functional groups, which have their own reactivity and selectivity. These crosslinkers can be used in a one step process or can be used to create activated proteins or antibodies, which can often be preserved and reacted with the second biomolecule in a separate step.

Heterobifunctional crosslinking reagents are often used for conjugate formation, where one protein or antibody is reacted with an amine reactive group (such as NHS or Sulfo-NHS), and the other protein is reacted with the other end of the crosslinker, which has a different selectivity (such as a maleimide group). These crosslinkers have been used in the formation of hapten carrier proteins and in preparing enzyme-antibody conjugates.

Due to their dual selectivity, heterobifunctional protein crosslinkers are extremely useful chemical tools for making a wide range of products and solving various research questions. CovaChem proudly manufacturers some of the highest quality heterobifunctional crosslinkers in the world. See how CovaChem can assist you with these chemicals or custom chemical crosslinkers by contacting our technical support team.


  • 4-Azidobenzoic acid

    p-Azidobenzoic acid is a chemical reagent that can be utilized to introduce a photoreactive (azide) group to a protein or other compound.

    Package Sizes: 1 gram and 5 grams

    Product Numbers: 14108-1 and 14108-5

    Product Name: 4-azidobenzoic acid

    Alternative Names: p-azidobenzoic acid; 4-ABA; HSAB acid

    Specifications

    Appearance: White Solid

    Purity: is ≥ 97%

    Storage: 4 °C, protected from light

     

    Properties Physical State: Solid

    CAS#: 6427-66-3

    Chemical Formula: C7H5N3O2S

    Molecular Weight: 163.13

    Tariff Code: 3824.90.9290

    $39.00
  • BMPS Crosslinker

    3-Maleimidopropionic acid N-hydroxysuccinimide ester (BMPS) is a heterobifunctional crosslinker with a spacer length of 5.9 Å. BMPS contains an amine reactive, NHS ester, and a maleimide group which is reactive towards sulfhydryl functional groups.

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 13401-100 and 13401-1

    Product Name: BMPS Crosslinker

    Alternative Names: BMPS; N-(3-Maleimidopropionyloxy)succinimide, N-Maleoyl-β-alanine N’-hydroxysuccinimide; N-Maleoyl-β-alanine N-hydroxysuccinimide ester, N-Succinimidyl 3-maleimidopropionate; C12H12N2O6

    Specifications

    Appearance: White Solid

    Purity: is ≥ 95% by quantitative NMR

    Storage: 4 °C, protected from moisture

     

    Properties Physical State: Solid

    CAS#: 55750-62-4

    Chemical Formula: C12H12N2O6

    Molecular Weight: 266.21

    Spacer length: 5.9 Å

    Melting Point: 168-170 °C (Lit)

    Country of Origin: USA

    Tariff Code: 2928.00.5000


    Additional Product Information:
    BMPS Product Information Bulletin
    BMPS Crosslinker MSDS
    $89.00
  • EMCS Crosslinker

    3-Maleimidocaproic acid N-hydroxysuccinimide ester (EMCS) is a heterobifunctional crosslinker with a spacer length of 9.4 Å. EMCS contains an amine reactive, NHS ester, and a maleimide group which is reactive towards sulfhydryl functional groups.

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 13402-100 and 13402-1

    Product Name: EMCS Crosslinker

    Alternative Names: EMCS; 6-Maleimidocaproic acid NHS; 6-Maleimidocaproic acid N-hydroxysuccinimide ester; 6-Maleimidocaproic acid N-succinimidyl ester, N-(ε-Maleimidocaproyloxy)succinimide, N-Succinimidyl 6-maleimidocaproate; C14H16N2O6

    Specifications

    Appearance: White Solid

    Purity: is ≥ 92% by quantitative NMR

    Storage: 4 °C, protected from moisture

     

    Properties Physical State: Solid

    CAS#: 55750-63-5

    Chemical Formula: C14H16N2O6

    Molecular Weight: 308.29

    Spacer length: 9.4 Å

    Melting Point: 68-70 °C (Lit)

    Country of Origin: USA

    Tariff Code: 2928.00.5000

    $162.00
  • GMBS Crosslinker

    4-Maleimidobutyric acid N-hydroxysuccinimide ester (GMBS) is a heterobifunctional crosslinker with a spacer length of 7.4 Å. GMBS contains an amine reactive, NHS ester, and a maleimide group which is reactive towards sulfhydryl functional groups.

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 13403-100 and 13403-1

    Product Name: GMBS Crosslinker

    Alternative Names: GMBS; N-[ γ -Maleimidobutyrloxy]succinimide ester; 4-Maleimidobutyric acid N-succinimidyl ester; N-Succinimidyl 4-maleimidobutyrate, GMBS; 80307-12-6; C12H12N2O6

    Specifications

    Appearance: White Solid

    Purity: is ≥ 90% by quantitative NMR

    Storage: 4 °C, protected from moisture

     

    Properties Physical State: Solid

    CAS#: 80307-12-6

    Chemical Formula: C12H12N2O6

    Molecular Weight: 280.24

    Spacer length: 7.4 Å

    Melting Point: 123-128 °C (Lit)

    Country of Origin: USA

    Tariff Code: 2928.00.5000

    $142.00
  • LC-SPDP Crosslinker

    Succinimidyl 6-[3-(2-pyridyldithio)propionamido] hexanoate (LC-SPDP) is a heterobifunctional crosslinker that is amino reactive on one end and sulfhydryl reactive on the other end. LC-SPDP contains a long spacer arm (15.7 Å)for increased molecular flexibility and a reducible disulfide, which is allows for cleavage of the crosslinker after crosslink formation.

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 13404-100 and 13404-1

    Product Name: LC-SPDP Crosslinker

    Alternative Names: LCSPDP; LC-SPDP; Succinimidyl 6-[3-(2-pyridyldithio)propionamido] hexanoate; Succinimidyl 6-[3-(2-pyridyldithio)-propionamido)hexanoate; C18H23N3O5 S2

    Specifications

    Appearance: White Solid

    Purity: is ≥ 90% by quantitative NMR

    Storage: 4 °C, protected from moisture

     

    Properties Physical State: Solid

    CAS#: N/A

    Chemical Formula: C18H23N3O5 S2

    Molecular Weight: 425.52

    Spacer length: 15.7 Å

    Melting Point: 80-84 °C (Lit)

    Country of Origin: USA

    Tariff Code: 2933.99.7900

    $197.00
  • MBS Crosslinker

    MBS is a heterobifunction crosslinker that contains a maleimide group which is reactive towards sulfhydryls and an NHS ester, useful for targeting amine groups.

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 13405-100 and 13405-1

    Product Name: MBS Crosslinker

    Alternative Names: MBS; m-Maleimidobenzoyl N-hydroxysuccinimide; CAS 58626-38-3; m-Maleimidobenzoyl NHS ester; 3-Maleimidobenzoic acid N-hydroxysuccinimide ester; C15H10N2O6

    Specifications

    Appearance: White Solid

    Purity: is ≥ 90% by quantitative NMR

    Storage: 4 °C, protected from moisture

     

    Properties Physical State: Solid

    CAS#: 58626-38-3

    Chemical Formula: C15H10N2O6

    Molecular Weight: 314.25

    Spacer length: 9.9 Å

    Melting Point: 175-178 °C (Lit)

    Country of Origin: USA

    Tariff Code: 2925.19.4200

    $59.00
  • PDPH Crosslinker

    3-(2-Pyridyldithio)propionyl hydrazide (PDPH) is a heterobifunctional crosslinker with specific reactivities toward carbohydrates and disulfides. PDPH creates crosslinks that are cleavable with DTT or TCEP.

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 13406-100mg and 13406-1g

    Product Name: PDPH Crosslinker

    Alternative Names: PDPH Crosslinker; 3-(2-Pyridyldithio)propionyl hydrazide; SPDP hydrazide; C8H11S2O

    Specifications

    Appearance: White to Off-White Solid

    Purity: is ≥ 90% by quantitative NMR

    Storage: 4 °C, protected from moisture

     

    Properties Physical State: Solid

    CAS#: N/A

    Chemical Formula: C8H11N3 S2O

    Molecular Weight: 229.32

    Spacer length: 9.2 Å

    Melting Point: 92-93 °C (Lit)

    Country of Origin: USA

    $119.00
  • SBA Crosslinker

    Succinimidyl bromoacetate (SBA) is a heterobifunctional crosslinker with a short spacer arm (1.5 Å). SBA is reactive towards amino groups and sulfhydryl groups. .

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 13407-100 and 13407-1

    Product Name: SBA Crosslinker

    Alternative Names: SBA; SBA Crosslinker; SBA NHS; Succinimidyl bromoacetate; 42014-51-7; C6H6NO4Br; NHS Bromoacetate; Bromoacetic acid N-hydroxysuccinimide ester; Bromoacetic acid NHS ester; C6H6BrNO4

    Specifications

    Appearance: White to Off-White Solid

    Purity: is ≥ 95% by quantitative NMR

    Storage: 4 °C, protected from moisture

     

    Properties Physical State: Solid

    CAS#: 42014-51-7

    Chemical Formula: C6H6BrNO4

    Molecular Weight: 236.02

    Spacer length: 1.5 Å

    Melting Point: 115-116 °C (Lit)

    Country of Origin: USA

    Tariff Code: 2933.99.9700

    $35.00
  • SIA Crosslinker

    Succinimidyl iodoacetate (SIA) is a heterobifunctional protein crosslinker with a short spacer arm (1.5 Å). SIA is reactive towards amino groups and sulfhydryl groups. SIA is a protein crosslinker commonly used to couple lysines on one protein and cysteine residues on another protein or antibody.

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 13408-100 and 13408-1

    Product Name: SIA Crosslinker

    Alternative Names: SIA; Succinimidyl iodoacetate; NHS Iodoacetate; SIA NHS; 39028-27-8; C6H6NO4I; Iodoacetic acid NHS ester; amino sulfhydryl reactive crosslinker; thiol reactive crosslinker; C6H6INO

    Specifications

    Appearance: White to Off-White Solid

    Purity: is ≥ 95% by quantitative NMR

    Storage: 4 °C, protected from moisture

     

    Properties Physical State: Solid

    CAS#: 39028-27-8

    Chemical Formula: C6H6INO

    Molecular Weight: 237.00

    Spacer length: 1.5 Å

    Melting Point: 148 °C (Lit)

    Country of Origin: USA

    Tariff Code: 2925.19.9100

    $49.00
  • SMCC Crosslinker

    SMCC is a heterobifunctional protein crosslinker. SMCC is commonly used to prepare antibody-enzyme conjugates. This molecule contains an amine reactive NHS ester on one end, and a maleimide functional group that can be utilized to react specifically with sulfhydryl (-SH) moeities. The maleimide functional group does not readily react with amino group (-NH2) as found on lysine residues, thus maleimide activated conjugates can be readily formed and preserved for later utilization.

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 13409-100mg and 13409-1g

    Product Name: SMCC Crosslinker

    Alternative Names: SMCC; SMCC Crosslinker; 64987-85-5; 4-(N-Maleimidomethyl)cyclohexanecarboxylic acid N-hydroxysuccinimide ester; N-Succinimidyl 4-(maleimidomethyl)cyclohexanecarboxylate; C16H18N2O6

    Specifications

    Appearance: White Solid

    Purity: is ≥ 90% by quantitative NMR

    Storage: 4 °C, protected from moisture

     

    Properties Physical State: Solid

    CAS#: 64987-85-5

    Chemical Formula: C16H18N2O6

    Molecular Weight: 334.32

    Spacer length: 8.3 Å

    Melting Point: 180-182 °C (Lit)

    Country of Origin: USA

    Tariff Code: 2928.00.5000


    Additional Product Information:
    SMCC Crosslinker Product Information Bulletin
    SMCC Crosslinker MSDS
    $88.00
  • SPDP Crosslinker

    N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP Crosslinker) is a cleavable heterobifunctional crosslinker. SPDP contains a dithiopyridyl group which is reactive towards cyteines (-SH groups) and an NHS group, which is useful when targetting primary amines (-NH2 groups). SPDP also contains a disulfide linkage in its spacer arm, which is cleavable with reducing agent, such as DTT, TCEP, among a few others carried by CovaChem.

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 13411-100mg and 13411-1g

    Product Name: SPDP Crosslinker

    Alternative Names: SPDP; SPDP Crosslinker; 68181-17-9; N-succinimidyl-3-(2-pyridyldithio)propionate; 3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester; 3-(2-Pyridyldithio)propionic acid NHS ester; C12H12N2O4S2

    Specifications

    Appearance: White Solid

    Purity: is ≥ 95% by quantitative NMR

    Storage: 4 °C, protected from moisture

     

    Properties Physical State: Solid

    CAS#: 68181-17-9

    Chemical Formula: C12H12N2O10

    Molecular Weight: 312.36

    Spacer length: 6.4 Å

    Melting Point: 84-86 °C (Lit)

    Country of Origin: USA

    Tariff Code: 2933.99.7900


    Additional Product Information:
    SPDP Crosslinker Product Information Bulletin
    SPDP Crosslinker MSDS
    $109.00
  • Sulfo SANPAH Crosslinker

    Product Name: Sulfo-SANPAH Crosslinker


    Sulfo-SANPAH is a water-soluble, heterobifunctional crosslinker with a photo-reactive nitrophenyl azide group on one end and an amine (-NH2) functional group on the other. Sulfo-SANPAH is soluble in organic solvents, such as DMF or DMSO as well as in aqueous buffers.

    Package Sizes: 5 x 5 mg, 100 mg and 1 gram

    Product Numbers: 13414-5x5, 13414-100 and 13414-1

    Alternative Names: Sulfo-SANPAH; Sulfo SANPAH; N-Sulfosuccinimidyl-6-(4'-azido-2'nitrophenylamino) hexanoate; nitrophenyl azide sulfo NHS ester; Sulfo-NHS-6-(4'-azido-2'nitrophenylamino) hexanoate; C16H17N6NaO9S

    Specifications

    Appearance: Red-Orange Solid

    Purity: is ≥ 90% by quantitative NMR

    Storage: 4 °C, protected from moisture

     

    Properties Physical State: Solid

    Chemical Formula: C16H17N6NaO9S

    Molecular Weight: 492.40

    Spacer length: 18.2 Å

    Country of Origin: USA

    Additional Product Information:
    Sulfo-SANPAH Product Information Bulletin
    Sulfo-SANPAH MSDS

    $109.00
  • Sulfo-LC-SPDP Crosslinker

    Sulfosuccinimidyl 6-(3'-[2-pyridyldithio]-propionamido)hexanoate (Sulfo-LC-SPDP Crosslinker) is a water soluble, cleavable, heterobifunctional protein crosslinker. Sulfo-LC-SPDP contains a dithiopyridyl group which is reactive towards cyteines (-SH groups) and an NHS group, which is useful when targetting primary amines (-NH2 groups). SPDP also contains a disulfide linkage in its spacer arm, which is cleavable with reducing agent, such as DTT, TCEP, among a few others carried by CovaChem.

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 13411-100mg and 13411-1g

    Product Name: SPDP Crosslinker

    Alternative Names: Sulfo-LC-SPDP; Sulfo-LC-SPDP Crosslinker; Sulfosuccinimidyl 6-[3-(2-pyridyldithio)propionamido] hexanoate; C18H22N3NaO8S3

    Specifications

    Appearance: White Solid

    Purity: is ≥ 90% by quantitative NMR

    Storage: 4 °C, protected from moisture

     

    Properties Physical State: Solid

    Chemical Formula: C18H22N3NaO8S3

    Molecular Weight: 527.57

    Spacer length: 15.7 Å

    Country of Origin: USA

    Tariff Code: 2933.99.7900

    $298.00
  • Sulfo-MBS Crosslinker

    Sulfo-MBS is a water soluble, heterobifunctional protein crosslinker that contains a sulfhydryl-reactive maleimide group, and a Sulfo-NHS ester, useful for targeting amine groups. p>

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 13413-100 and 13413-1

    Product Name: Sulfo-MBS Crosslinker

    Alternative Names: Sulfo-MBS; m-Maleimidobenzoyl N-hydroxysulfosuccinimide; m-Maleimidobenzoyl Sulfo-NHS ester; 3-Maleimidobenzoic acid N-hydroxysulfosuccinimide ester; C15H9N2NaO9S

    Specifications

    Appearance: White Solid

    Purity: ≥ 90% by quantitative NMR

    Storage: 4 °C, protected from moisture

     

    Properties

    Physical State: Solid

    Chemical Formula: C15H9N2NaO9S

    Molecular Weight: 416.29

    Spacer length: 9.9 Å

    Country of Origin: USA

    Tariff Code: 2925.19.4200

    $129.00
  • Sulfo-SMCC

    Sulfo-SMCC is a water-soluble heterobifunctional protein crosslinker. Sulfo-SMCC protein crosslinker contains an amine reactive Sulfo-NHS ester on one end, which increases its water solubility, and a maleimide functional group that can be utilized to react specifically with cysteines or sulfhydryl (-SH) groups. The maleimide functional group does not readily react with lysine or amino group (-NH2), thus maleimide activated conjugates can be readily prepared for later utilization. This makes Sulfo-SMCC ideal for preparing antibody-enzyme conjugates.

    The heterobifunctional nature of Sulfo-SMCC gives it a wide range of uses for both small molecule reactions and large biomolecule applications. Sulfo-SMCC is perhaps one of the most common reagents used in the creation of enzyme labelled antibodies, other bioconjugates, and activated antibodies available for further reactivity. As opposed to other maleimide activated antibodies, those made with SMCC or Sulfo SMCC crosslinkers are often more stable, due to the inductive stabilizing effects of the cyclohexyl ring moeity.

    Package Sizes: 100 mg and 1 gram

    Product Numbers: 13415-100mg and 13415-1g

    Product Name: Sulfo SMCC Crosslinker

    Alternative Names: Sulfo-SMCC; SSMCC; Sulfo-SMCC Crosslinker; 4-(N-Maleimidomethyl)cyclohexane-1-carboxylic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt; 92921-24-9; Sulfo smcc; Sulfosmcc; C16H17N2NaO9S


    Specifications

    Sulfo-SMCC NMR Spectrum

    Appearance: White Solid

    Purity: is ≥ 90% by quantitative NMR

    Storage: 4 °C, protected from moisture

     

    Properties

    Physical State: Solid

    CAS#: 92921-24-9

    Chemical Formula: C16H17N2NaO9S

    Molecular Weight: 436.37

    Spacer length: 6.4 Å

    Country of Origin: USA

    Tariff Code: 2933.99.9700


    Additional Product Information:
    Sulfo-SMCC Product Information Bulletin
    Sulfo-SMCC Crosslinker MSDS

    $128.00