DTSSP Crosslinker

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DTSSP Crosslinker
Package Sizes: 100 mg
SKU: 13304-100
WEIGHT: 0.20
DTSSP Crosslinker
Package Sizes: 1 gram
SKU: 13304-1
WEIGHT: 0.20

DTSSP is a cleavable protein crosslinking reagent that contains a disulfide moeity in its eight atom spacer arm. DTSSP is a membrane impermeable, homobifunctional crosslinker that is reactive towards amine (amino) functional groups. In the pH range of 7-9, this reaction is particularly selective for primary amine groups, resulting in the formation of stable amide linkages. Phosphate buffers and phosphate buffered saline are typically used for crosslinking reactions involving DTSSP, although others non-amine containing buffers may also be used.

Once the reaction between DTSSP and proteins or peptides is complete, the disulfide-containing crosslinked protein can be cleaved via reduction of the crosslinkers disulfide bond using a reducing agent, such as 2-mercaptoethanol (CovaChem 11301-10x1) or DTT (CovaChem 11302-10x5). CovaChem's technical support team is readily available to assist with any individual optimization.

Dithiobis(sulfosuccinimidyl propionate) (DTSSP) is the water soluble version of Lomant's Reagent (DSP). Both DSP and DTSSP have been used extensively in protein crosslinker applications as well as other scientific applications involving forming a stable linkages between the disulfide group and metal surfaces, while leaving the NHS functional groups available to react with other nucleophiles. This application can be useful for immobilizing proteins or other molecules to the surface of gold beads or other foil.

Due to the lipophobic nature of the sulfonate groups on DTSSP, this crosslinker does not permeate the cell membrane. This feature makes DTSSP particularly useful for crosslinking membrane proteins on the surface of the cell without interfering with intercellular proteins.

Package Sizes: 100 mg and 1 gram

Product Numbers: 13304-100 and 13304-1

Product Name: DTSSP Crosslinker

Alternative Names: DTSSP; 3,3'-Dithiobis(sulfosuccinimidyl propionate); Dithiobis(sulfosuccinimidyl propionate); 81069-02-5; cleavable Sulfo-NHS crosslinker; DTSSP Protein Crosslinker


Appearance: White Solid

Purity: is ≥ 90% Storage: 4 °C, protected from moisture


Properties Physical State: Solid

CAS#: 81069-02-5

Chemical Formula: C14H14N2Na2O14S4

Molecular Weight: 608.51

Spacer length: 12.0 Å

Additional Product Information:

DTSSP Product Information Bulletin
DTSSP Crosslinker MSDS

  • BS2G Crosslinker

    BS2G (Sulfo-DSG) is a water soluble amine (amino) reactive homobifunctional crosslinker.
  • DCC (Dicyclohexylcarbodiimide)

    N,N''-Dicyclohexylcarbodiimide (DCC) is a zero length crosslinker. Dicyclohexylcarbodiimide is a coupling reagent used to couple carboxylic acids (COOH) to primary amines (-NH2).
  • DHB MALDI Matrix

    2,5-Dihydroxybenzoic acid (DHB) is a MALDI matrix used in the MALDI-MS of proteins, peptides, synthetic polymers, lipids and carbohydrates.
  • DST Crosslinker

    Disuccinimidyl tartrate (DST) is an amine reactive, water soluble, cleavable crosslinker. DST is a homobifunctional crosslinker that is reactive towards amines
  • EDC-HCL | EDAC-HCL | > 99%

    N-Ethyl-N'(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC-HCl) is a zero length protein crosslinker. Also known as EDAC-HCl, this reagent is often used with either Sulfo-NHS (CovaChem 13505) or NHS (CovaChem 13504). EDC functions by activating the carboxyl region of the protein or other molecule, and coupling it to an amine or other nucleophilic functional group.
  • GMBS Crosslinker

    4-Maleimidobutyric acid N-hydroxysuccinimide ester (GMBS) is a heterobifunctional crosslinker with a spacer length of 7.4 Å. BMPS contains an amine reactive, NHS ester, and a maleimide group which is reactive towards sulfhydryl functional groups.
  • HABA MALDI Matrix

    HABA MALDI Matrix
  • HPLC Grade Acetone

    CovaChem's HPLC Grade Acetone is a high purity solvent ideally suited for HPLC applications and general laboratory use.