Zero Length Crosslinkers

  • DCC (Dicyclohexylcarbodiimide)

    DCC Product Description

    N,N'-Dicyclohexylcarbodiimide (DCC) is a zero length coupling reagent. DCC has both biochemical and synthetic applications. This reagent can be used to couple primary amines to carboxyllic acid functional groups. DCC is soluble in many organic solvents, while the DCU by product is generally insoluble and easily removed.

    DCC Coupling Reaction Mechanism

    Package Sizes: 5 grams and 25 grams

    Product Numbers: 13502-5; 13502-25

    Product Name: DCC Crosslinker

    Alternative Names: DCC; 1,1'-Dicyclohexylcarbodiimide; N,N'-Dicyclohexylcarbodiimide; C13H22N2; 538-75-0


    Specifications

    Appearance: White Solid

    Purity: is ≥ 99%

    Storage: Room Temperature, Protected From Moisture

     

    Properties

    Physical State: Solid

    CAS#: 538-75-0

    Chemical Formula: C13H22N2

    Molecular Weight: 206.33

    Spacer length: 0 Å

    Melting Point: 34 - 35 °C (Lit)

    Tariff Code: 2925.19.4200


    $19.00
  • EDC-HCL | EDAC-HCL | > 99%

    N-Ethyl-N'(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC-HCl) is a zero length protein crosslinker. Also known as EDAC-HCl, this reagent is often used with either Sulfo-NHS (CovaChem 13505) or NHS (CovaChem 13504). EDC functions by activating the carboxyl region of the protein or other molecule, and coupling it to an amine or other nucleophilic functional group.

    Package Sizes: 3x10 mg, 100 mg, 1 gram, 25 grams, and 100 grams

    Product Numbers: 13503-3x10, 13503-100, 13503-1, 13503-25, 13503-100B

    Product Name: EDC-HCl Crosslinker

    Alternative Names: EDC; EDC-HCl; EDAC; EDAC-HCl; 3-(ethyliminomethyleneamino)-N,N-dimethylpropan-1-amine; N-Ethyl-N'(3-dimethylaminopropyl)carbodiimide hydrochloride; EDC hydrochloride; C8H18ClN3; 25952-53-8

    Specifications

    Appearance: White Solid

    Purity: is ≥ 99%

    Storage: -20 °C, protected from moisture

     

    Properties Physical State: Solid

    CAS#: 25952-53-8

    Chemical Formula: C8H18ClN3

    Molecular Weight: 191.70

    Spacer length: 0 Å

    Melting Point: 110-116 °C (Lit)

    Country of Origin: USA

    Tariff Code: 2925.29.9000


    Online Technical Resources:

    EDC Product Information Bulletin

    Protocol for Coupling of Two Proteins with EDC and Sulfo-NHS

    $19.00
  • N,N'-Carbonyldiimidazole (CDI)

    CDI Product Description

    N,N'-Carbodiimide (CDI) is a zero length crosslinking reagent used to couple primary amines to carboxyllic acid functional groups. CDI reacts with the carboxylate ion (COO-) of proteins or other organic compounds, forming a reactive intermediate that is subsequently reacted with an amino group (-NH2). This reagent can be used to couple two proteins, and is commonly used in protein immobilization to a solid support or membrane. Conveniently, CDI can be used in both aqueous and organic solvent systems.

    CDI Reaction Mechanism

    Package Sizes: 5 grams and 25 grams

    Product Numbers: 13501-5; 13501-25

    Product Name: CDI Crosslinker

    Alternative Names: CDI; 1,1'-Carbonyldiimidazole; N,N'-Carbonyldiimidazole; C7H6N4O; 530-62-1


    Specifications

    Appearance: White Solid

    Purity: is ≥ 99%

    Storage: 4 °C, protected from moisture

     

    Properties

    Physical State: Solid

    CAS#: 530-62-1

    Chemical Formula: C7H6N4O

    Molecular Weight: 162.15

    Spacer length: 0 Å

    Melting Point: 118 - 120 °C (Lit)

    Tariff Code: 2933.29.9000


    $23.00
  • N-Hydroxysuccinimide (NHS)

    N-Hydroxysuccinimide (NHS) is used in conjunction with EDC-HCl (CovaChem 13503). NHS is used as a catalyst in the zero-length crosslinking of proteins with EDC. N-Hydroxysuccinimide functions by stabilizing the protein-EDC intermediate, thus allowing for a more efficient crosslinking reaction.

    Package Sizes: 25 grams

    Product Numbers: 13504-25

    Product Name: N-Hydroxysuccinimide

    Alternative Names: Hydroxysuccinimide; NHS; N-Hydroxysuccinimide; 1-Hydroxy-2,5-pyrrolidinedione; HOSu; 6066-82-6; C4H5NO3

    Specifications

    Appearance: White Solid

    Purity: is ≥ 98% by quantitative NMR

    Storage: Room Temperature, protected from moisture

    Properties

    Properties Physical State: Solid

    CAS#: 6066-82-6

    Chemical Formula: C4H5NO3

    Molecular Weight: 115.09

    Country of Origin: USA

    Tariff Code: 2928.00.5000


    Additional Product Information:
    NHS Product Information Bulletin
    N-Hydroxysuccinimide (NHS) MSDS
    $30.00
  • Sulfo-NHS

    N-Hydroxysulfosuccinimide sodium salt (Sulfo-NHS) is used in conjunction with EDC-HCl (CovaChem 13503). Sulfo-NHS is used as a catalyst in the zero-length crosslinking of proteins with EDC. Sulfo-NHS functions by stabilizing the protein-EDC intermediate, thus allowing for a more efficient crosslinking reaction.

    Package Sizes: 500 mg, 1 gram, 5 grams

    Product Numbers: 13405-500, 13405-1, and 13405-5

    Product Name: Sulfo-NHS

    Alternative Names: Hydroxy-2,3-dixopyrrolidine-3-sulfonic acid sodium salt; Sulfo-NHS; SNHS; N-Hydroxysulfosuccinimide sodium salt; 106627-54-7; C4H4NNaO6S

    Specifications

    Appearance: White Solid

    Purity: ≥ 99% by quantitative NMR

    Storage: 4 °C, protected from moisture

    Properties

    Properties Physical State: Solid

    CAS#: 106627-54-7

    Chemical Formula: C4H4NNaO6 S

    Molecular Weight: 217.13

    Country of Origin: USA

    Tariff Code: 2928.00.5000


    Additional Product Information:

    Sulfo NHS Product Information Bulletin
    Sulfo NHS Protein Coupling Protocol
    Sulfo NHS Sulfosuccinimide MSDS

    $79.00